Werth's roster spot was taken by outfielder Eury Perez, who was recalled from Triple-A Syracuse before Washington hosted the Chicago Cubs on Saturday.
Harper posted two images to Twitter, one of his toe with no nail and very bloody. The other was of a full toenail not attached to his foot at all.
Harper was expected to play in today, Saturday's, game by manager Davey Johnson of the Nationals but, Harpers toe was still 'tender'.
After Friday night's 7-3 victory over Chicago, Werth said: "It's one of those things I feel bad; the team has been playing with 24 guys for over a week now. We feel like we're close and I feel like it's really not that severe. But it's one of those things you go out there with the way it is, and you might make it severe pretty quickly. Erring on the side of caution, but at the same time at this point I think it's necessary.
The replacement for Werth, Perez, has been excelling in triple-A for Syracuse with 17 runs, a .346 batting average, as well as 7 steals all in only 23 games.
Perez appeared in only 13 games for the Washington Nationals in the 2012 season getting only 5 at bats with 1 hit.